BufferFish is a web application for scientists working in Chemistry and Biology that will help them to save time and avoid errors in repetitive daily tasks such as calculations, images, and charts labeling and analysis.

For now, you can use the first available BufferFish feature - Concentration Calculator - to make calculations to prepare chemical solutions in the lab. Check for more details here.

In the future releases more features will be available which will include:

Is there any feature you always wanted to have which will make your life in the lab easier? You can let us know here!

The Concentration Calculator is a complete automated calculator that will calculate the concentration your solution needs.

It is software designed to simplify your work in the laboratory when you want to prepare any liquid chemical mixture, and you want to know how much of each ingredient to take. You can use the concentration calculator to make calculations for any chemical solution you are preparing in the lab, either by making an HPLC buffer, preparing a protein sample, or setting up a PCR reaction.

The Unit Converter is a conversion calculator specially designed for those who work in the wet lab. You can automatically convert units between mg/ml, molar, and % concentration with no need to remember formulas.

The Protein Calculator is a complete automated calculator that will allow you to get the Molecular Weight and extinction coefficient of your protein’s sequences. You can introduce a sequence manually or upload your FASTA file with multiple sequences and calculate all of them at the same time with just one click.

The Gel Analysis is a BufferFish feature thought to reduce the iterations when analyzing and sharing your gel results. When uploading your gel analysis results, the software will automatically detect the lanes and bands in the image and will provide a space where labels can be placed.

Results can be saved in an interactive format, so you don’t need to go to the original image to make changes.

This feature will help you to easily visualize and label your chromatograms. Results can be saved in an interactive format, so you don’t need to go to the original file to make changes.

It can also be used with other types of charts.

For now we have just released the Concentration Calculator. If you want to receive updates when new features are released, stay tuned here.

Currently we have released a BufferFish free beta version. Once the beta phase is over, you will be able to enjoy a 30 days free trial. If you want to know more about BufferFish Pricing, please let us know here.

No, BufferFish is a web application that can be used whenever and wherever you are with no need to download it.

No, you don’t need to be registered to use it. However, you must be registered and logged in if you want to Import/Export your BufferFish results.

Only emails that have been confirmed during the registration process are able to receive the email to recover the password. If you didn’t confirm your password, please contact us here.

Yes, if you want to delete your BufferFish account, contact us and our team will delete it for you. Bear in mind that if you don’t have a BufferFish account, you won’t be able to import/export any of your calculation results.

At the moment the web app is only available for desktop devices. Please let us know if you would like to use it from a mobile device. Please note that you can’t interact with the app using a touchscreen on your desktop device.

With the Concentration Calculator you can create recipes for your chemical solutions. It’s like you always did with Excel, but easier as you don’t need to remember formulas for calculations. The only thing you need to do is to input your components’ data (e.g. initial concentrations or molecular weight) and the final concentrations of all the components you want to have in your recipe.

You can add up to 30 ingredients in your recipe.

Yes, you can choose if your ingredient is liquid or solid, and you can use both to make the calculation.

When mixing liquids, volume doesn't always add up. For example, this happens when mixing water and ethanol. Remember that if you are dealing with such liquids, the amount of diluent calculated with the Concentration Calculator will not be correct. In such a case we recommend you to use the measuring cylinder to fill the diluent precisely till the desired volume. Alternatively, you can measure such liquids by weight for getting higher precision results.

When you are dealing with very viscous liquids (for example, 99 % glycerol), it is difficult to measure volume of such liquids with high precision, especially if you are dealing with small volumes (for example, in milliliter range). In such cases it is highly recommended to measure the amount of liquid by weight for getting higher precision results.

You can use all the most commonly used concentration units for your calculations (molar, mg/mL, %, U/mL, and even x-times dilution factor) in the same recipe.

If you think you need other units different from these ones for your daily experiments, please let us know here.

Yes, The calculator “knows” how to convert metric prefixes automatically. Just enter the units exactly as in your protocol.

Yes, you can download your recipe in PDF, .json, or directly print it. To be able to save your recipe you must be logged into your BufferFish account.

A .json format is a file format that will allow you to save your BufferFish results for future editing.

Once you have downloaded your results in .json format, you can upload them again to BufferFish and edit them, making the process of creating a recipe much easier and faster.

Yes, you can edit the recipes that you have previously saved as a .json file. All you need to do is upload your .json file and the calculator will show the results ready for you to edit them. To be able to import/export your .json file, you must be logged in.

No, you can’t recover a deleted recipe if you haven’t previously downloaded it.

In the number field of the Concentration Calculator, only numbers (0-9) and dot (.), and commas (,) are allowed as input values. Note that if a comma is inserted, it will be automatically transformed into a dot.

In numeric fields, you can insert up to 10 digits before the dot (whole number part), and up to 5 numbers after the dot (decimal part).

Note that the Concentration Calculator will automatically adjust the calculated amount to the metric prefix, which is comfortable to read. For example, you will see 2 mg instead of 0.002 g

Any calculated number will be rounded to two digits after the decimal dot following the standard rules (0-4 number rounded down, 5-9 number rounded up)